[Optimization of extraction technology for typhae pallen from tangwang mixture by central composite design-response surface method].

OBJECTIVE

To optimize the extraction process of Typhae Pollen from Tangwang Mixture.

METHODS

Using extraction rate and the content of isorhamnetin-3-O-neohesperidoside as the evaluation indicator, the extraction ways of Typhae Pollen was determined by comparing water and alcohol extraction technology. Among the water extraction technology, investigated the effect of single extraction and mix extraction, and whether Typhae Pollen wrappes during extracting. Taking ethanol concentration, the amount of solvent and extraction time as investigate factors, using the multiple linear regression and binomial formula fitting, extraction process was optimized by response surface method.

RESULTS

The optimum extraction technology was as follows: extracted 3 times with 8-fold the amount of 60% ethanol for 60 minutes each time.

CONCLUSION

The optimized ethanol extracted technology is reasonable and can be applied for the large-scale production.