Allan David S J, Lepin Eric J M, Braud Veronique M, O'Callaghan Christopher A, McMichael Andrew J
Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, OX3 9DS, UK.
J Immunol Methods. 2002 Oct 1;268(1):43-50. doi: 10.1016/s0022-1759(02)00199-0.
HLA-E, HLA-F, and HLA-G are human nonclassical MHC class Ib molecules. To study the function and identify potential ligands of these molecules, we constructed tetrameric complexes. In this brief review, we discuss the methods used to produce such tetramers and the interesting results they provided. HLA-E tetramers bound to natural killer (NK) cells and T cells, allowing the identification of CD94/NKG2 molecules as receptors for HLA-E. HLA-G tetramers interacted with immunoglobulin-like transcript-2 (ILT2) and ILT4 receptors, aiding the understanding of HLA-G function during pregnancy. Tetrameric complexes of HLA-F also bound to ILT2 and ILT4.
HLA - E、HLA - F和HLA - G是人类非经典的MHC Ib类分子。为了研究这些分子的功能并鉴定其潜在配体，我们构建了四聚体复合物。在这篇简短的综述中，我们讨论了用于产生此类四聚体的方法以及它们所提供的有趣结果。HLA - E四聚体与自然杀伤（NK）细胞和T细胞结合，从而能够鉴定出CD94/NKG2分子作为HLA - E的受体。HLA - G四聚体与免疫球蛋白样转录物2（ILT2）和ILT4受体相互作用，有助于理解妊娠期间HLA - G的功能。HLA - F的四聚体复合物也与ILT2和ILT4结合。
