Tong Yat-Ching, Cheng Juei-Tang, Wan Wang-Chuan
Department of Urology, College of Medicine, National Cheng Kung University, Tainan City, 70101, Taiwan, ROC.
Neurosci Lett. 2002 Sep 13;330(1):21-4. doi: 10.1016/s0304-3940(02)00713-9.
The study investigated the effect of a traditional Chinese herbal preparation 'Ba-Wei-Die-Huang-Wan' on the cholinergic function of the urinary bladder in diabetic rats, as well as its influence on the protein expression of muscarinic M(2) receptors. Wistar rats were divided into three groups: (1) control rats; (2) 2-week streptozotocin-induced diabetic rats; and (3) diabetic rats treated with Ba-Wei-Die-Huang-Wan 26 mg/kg thrice daily for 3 days. A dose-response study was performed for bladder muscle strip contractile response to a muscarinic agonist, arecaidine propargyl ester (APE). The amount of M(2) receptor protein in the bladder was measured by Western immunoblotting using monoclonal antibodies. The bladder contractile response to APE was significantly increased in the diabetic rats compared to the control. The M(2) receptor protein density was also significantly higher by 106% (P < 0.01, n = 8) in diabetes. Ba-Wei-Die-Huang-Wan treatment significantly lowered the plasma glucose level of the diabetic rats. The increases in contractile response to APE as well as the M(2) receptor protein density were partially reversed by the treatment. In conclusion, there was an over-expression of M(2) receptor resulting in hyper-contractility in the bladder of diabetic rats. Ba-Wei-Die-Huang-Wan significantly alleviated the alterations.
该研究调查了传统中药制剂“八味地黄丸”对糖尿病大鼠膀胱胆碱能功能的影响，以及其对毒蕈碱M(2)受体蛋白表达的影响。将Wistar大鼠分为三组：(1)对照大鼠；(2)经链脲佐菌素诱导2周的糖尿病大鼠；(3)用八味地黄丸26mg/kg每日三次治疗3天的糖尿病大鼠。对膀胱肌肉条对毒蕈碱激动剂槟榔碱炔丙酯(APE)的收缩反应进行了剂量反应研究。使用单克隆抗体通过Western免疫印迹法测量膀胱中M(2)受体蛋白的量。与对照组相比，糖尿病大鼠对APE的膀胱收缩反应显著增加。糖尿病组中M(2)受体蛋白密度也显著升高106%（P<0.01，n=8）。八味地黄丸治疗显著降低了糖尿病大鼠的血糖水平。治疗部分逆转了对APE收缩反应以及M(2)受体蛋白密度的增加。总之，糖尿病大鼠膀胱中存在M(2)受体过表达，导致膀胱过度收缩。八味地黄丸显著减轻了这些改变。
