秋水仙碱对蛙皮全层上皮及分离细胞通透性的影响。

Colchicine effect on the permeability of the whole epithelium and of isolated cells of frog skin.

作者信息

Svelto M, Cremaschi D, Lippe C

出版信息

J Bioenerg Biomembr. 1979 Dec;11(5-6):103-12. doi: 10.1007/BF00743198.

Abstract

The effect of 2 X 10(-5) M colchicine on epithelial cells isolated from frog skins was investigated. Three hours of treatment with colchicine did not change either Na+ and K+ content of isolated cells or nonelectrolyte permeability. When ADH (50 mU/ml) was added, thiourea uptake values became greater than without the hormone; the same values were found in the cells previously treated with colchicine. Na+ transepithelial transport, measured by means of short-circuit current, was inhibited by the antimitotic agent both under control conditions and after ADH stimulation. These results support the view that colchicine does not directly affect ADH action on membrane permeability, but influences some mechanism that controls ADH action on transepithelial transport. Intercellular junctions appear to be the location of such a mechanism.

摘要

研究了2×10⁻⁵ M秋水仙碱对从蛙皮分离的上皮细胞的作用。用秋水仙碱处理三小时，既未改变分离细胞的Na⁺和K⁺含量，也未改变非电解质通透性。当加入抗利尿激素（ADH，50 mU/ml）时，硫脲摄取值比未加该激素时更高；在用秋水仙碱预先处理过的细胞中也发现了相同的值。在对照条件下以及在ADH刺激后，通过短路电流测量的Na⁺跨上皮转运均受到抗有丝分裂剂的抑制。这些结果支持这样一种观点，即秋水仙碱并不直接影响ADH对膜通透性的作用，而是影响某种控制ADH对跨上皮转运作用的机制。细胞间连接似乎是这种机制的所在位置。