植物乳杆菌中cspL基因的冷休克诱导涉及转录调控。
Cold shock induction of the cspL gene in Lactobacillus plantarum involves transcriptional regulation.
作者信息
Derzelle Sylviane, Hallet Bernard, Ferain Thierry, Delcour Jean, Hols Pascal
机构信息
Institut des Sciences de la Vie/Unité de Génétique, Université Catholique de Louvain, B-1348 Louvain-la-Neuve, Belgium.
出版信息
J Bacteriol. 2002 Oct;184(19):5518-23. doi: 10.1128/JB.184.19.5518-5523.2002.
Abstract
Fragments of the cspL promoter region were fused to the gusA reporter and reintroduced into Lactobacillus plantarum cells, either on multicopy plasmids or through single-copy chromosomal integration. beta-Glucuronidase activity and primer extension data demonstrate that the cspL promoter is induced in response to cold shock and that multicopy constructs quench the induction of the resident cspL gene.
摘要
将cspL启动子区域的片段与gusA报告基因融合，并通过多拷贝质粒或单拷贝染色体整合重新导入植物乳杆菌细胞中。β-葡萄糖醛酸酶活性和引物延伸数据表明，cspL启动子在冷休克响应中被诱导，并且多拷贝构建体会抑制常驻cspL基因的诱导。
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