起始密码子下游的序列对于大肠杆菌cspB的冷休克诱导至关重要。
A sequence downstream of the initiation codon is essential for cold shock induction of cspB of Escherichia coli.
作者信息
Etchegaray J P, Inouye M
机构信息
Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway, New Jersey 08854, USA.
出版信息
J Bacteriol. 1999 Sep;181(18):5852-4. doi: 10.1128/JB.181.18.5852-5854.1999.
Abstract
Cold shock induction of cspB has been shown to be primarily regulated at the mRNA level. Here, we demonstrate that the induction of cspB at low temperature also requires the translational cis-acting element called the downstream box (DB). Full induction of cspB at low temperature is achieved in the presence of both the Shine-Dalgarno sequence and DB. We propose that the DB sequence functions as a translational enhancer for the biosynthesis of CspB to bypass the inhibitory effect in translation caused by cold shock.
摘要
cspB的冷休克诱导已被证明主要在mRNA水平上受到调控。在此，我们证明低温下cspB的诱导还需要一种称为下游框（DB）的翻译顺式作用元件。在存在Shine-Dalgarno序列和DB的情况下，低温下cspB可实现完全诱导。我们提出，DB序列作为CspB生物合成的翻译增强子，以绕过冷休克在翻译中引起的抑制作用。
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