Departamento de Tecnología de Alimentos, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Carretera de La Coruña Km 7.5, 28040, Madrid, Spain.
Appl Microbiol Biotechnol. 2020 May;104(9):3797-3805. doi: 10.1007/s00253-020-10426-0. Epub 2020 Mar 4.
Lactobacillus species are attractive hosts for the expression of heterologous proteins, antigens, vaccines, and drugs due to their GRAS (generally recognized as safe) status. The bioengineering techniques open new possibilities of improving Lactobacillus strains. In this regard, the control of the gene expression in Lactobacillus strains through the adequate native or engineered promoters acquires a key role in the development of biotechnological applications and for their function as probiotic bacteria. Depending on the objective sought, the protein produced and the strain used, inducible or constitutive promoters can be chosen. Whereas, when a fine-tuning of gene expression is required, the development of synthetic promoter libraries could be the best approach. In this work, we revise the main constitutive and inducible natural promoters from Lactobacillus strains or from other genus that have been applied in Lactobacillus, as well as the few engineered promoters developed for these bacteria.
由于其 GRAS（通常被认为是安全的）地位，乳杆菌属是表达异源蛋白、抗原、疫苗和药物的有吸引力的宿主。生物工程技术为改善乳杆菌菌株开辟了新的可能性。在这方面，通过适当的天然或工程启动子来控制乳杆菌菌株中的基因表达，在开发生物技术应用和作为益生菌细菌的功能方面起着关键作用。根据所寻求的目标、所产生的蛋白质和所使用的菌株，可以选择诱导型或组成型启动子。然而，当需要精细调节基因表达时，合成启动子文库的开发可能是最佳方法。在这项工作中，我们回顾了已应用于乳杆菌的来自乳杆菌属或其他属的主要组成型和诱导型天然启动子，以及为这些细菌开发的少数工程启动子。
