Drapier Dominique, Girard-Bascou Jacqueline, Stern David B, Wollman Francis-André
UPR1261, IBPC, 13 rue Pierre et Marie Curie, 75005 Paris, France.
Plant J. 2002 Sep;31(6):687-97. doi: 10.1046/j.1365-313x.2002.01387.x.
We have characterized a nuclear mutation, mda1-ncc1, that affects mRNA stability for the atpA gene cluster in the chloroplast of Chlamydomonas. Unlike all nuclear mutations altering chloroplast gene expression described to date, mda1-ncc1 is a dominant mutation that still allows accumulation of detectable amounts of atpA mRNAs. At variance with the subset of these mutations that affect mRNA stability through the 5' UTR of a single chloroplast transcript, the mutated version of MDA1 acts on the coding region of the atpA message. We discuss the action of MDA1 in relation to the unusual pattern of expression of atpA that associates particularly short lived-transcripts with a very high translational efficiency.
我们鉴定了一种核突变，即mda1-ncc1，它影响衣藻叶绿体中atpA基因簇的mRNA稳定性。与迄今为止描述的所有改变叶绿体基因表达的核突变不同，mda1-ncc1是一种显性突变，仍能使可检测量的atpA mRNA积累。与通过单个叶绿体转录本的5'UTR影响mRNA稳定性的这些突变子集不同，MDA1的突变形式作用于atpA信息的编码区。我们讨论了MDA1的作用与atpA异常表达模式的关系，该模式将特别短命的转录本与非常高的翻译效率联系起来。
