Mata Alvaro, Boehm Cynthia, Fleischman Aaron J, Muschler George, Roy Shuvo
BioMEMS Laboratory, Department of Biomedical Engineering (ND20), The Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA.
J Biomed Mater Res. 2002 Dec 15;62(4):499-506. doi: 10.1002/jbm.10353.
Growth of human connective tissue progenitor cells (CTPs) was characterized on smooth and microtextured polydimethylsiloxane (PDMS) surfaces. Human bone-marrow-derived cells were cultured for 9 days under conditions promoting osteoblastic differentiation on smooth PDMS surfaces and on PDMS post microtextures that were 6 microm high and 5, 10, 20, and 40 microm in diameter, respectively. Glass tissue-culture dishes were used as controls. The number of viable cells was determined, and an alkaline phosphatase stain was used as a marker for osteoblastic phenotype. CTPs attached, proliferated, and differentiated on all surfaces. Cells on the smooth PDMS and control surfaces spread and proliferated as colonies in proximity to other cells and migrated in random directions, with cell process lengths of up to 80 microm. In contrast, cells on the PDMS post microtextures grew as sparsely distributed networks of cells, with processes, occasionally up to 300 microm, that appeared to interact with the posts. Cell counts revealed that there were fewer (50%) CTPs on the smooth PDMS surface than were on the glass control surfaces. However, there were consistently more (>144%) CTPs on the PDMS post textures than on the controls. In particular, the 10-microm-in-diameter posts (268%) exhibited a significantly (p < 0.05) greater cell number than did the smooth PDMS.
在光滑和具有微纹理的聚二甲基硅氧烷（PDMS）表面对人结缔组织祖细胞（CTP）的生长特性进行了研究。将人骨髓来源的细胞在促进成骨细胞分化的条件下，分别在光滑的PDMS表面以及直径分别为6微米高、5微米、10微米、20微米和40微米的PDMS微纹理表面上培养9天。使用玻璃组织培养皿作为对照。测定活细胞数量，并使用碱性磷酸酶染色作为成骨细胞表型的标志物。CTP在所有表面上均能附着、增殖并分化。光滑PDMS表面和对照表面上的细胞以集落形式在其他细胞附近扩散和增殖，并随机迁移，细胞突起长度可达80微米。相比之下，PDMS微纹理表面上的细胞生长为稀疏分布的细胞网络，其突起偶尔可达300微米，似乎与微纹理相互作用。细胞计数显示，光滑PDMS表面上的CTP比玻璃对照表面上的少（50%）。然而，PDMS微纹理表面上的CTP始终比对照表面上的多（>144%）。特别是，直径为10微米的微纹理表面（268%）的细胞数量比光滑PDMS表面显著（p<0.05）更多。
