Kang Pei-Leun, Lin Yu-Hsin, Settu Kalpana, Yen Ching-Shu, Yeh Chin-Yi, Liu Jen-Tsai, Chen Ching-Jung, Chang Shwu-Jen
Cardiovascular Surgery, Department of Surgery, Kaohsiung Veterans General Hospital, Kaohsiung 81362, Taiwan.
Department of Electrical Engineering, National Taipei University, New Taipei 23741, Taiwan.
Polymers (Basel). 2020 May 22;12(5):1186. doi: 10.3390/polym12051186.
This study focuses on preparation and valuation of the biodegradable, native, and modified gelatin film as screen-printing substrates. Modified gelatin film was prepared by crosslinking with various crosslinking agents and the electrode array was designed by screen-printing. It was observed that the swelling ratio of C-2, crosslinked with glutaraldehyde and EDC/NHS (1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide) was found to be lower (3.98%) than that of C-1 (crosslinked with only glutaraldehyde) (8.77%) and C-0 (without crosslinking) (28.15%). The obtained results indicate that the swelling ratios of both C-1 and C-2 were found to be lower than that of C-0 (control one without crosslinking). The Young's modulus for C-1 and C-2 was found to be 8.55 ± 0.57 and 23.72 ± 2.04 kPa, respectively. Hence, it was conveyed that the mechanical strength of C-2 was found to be two times higher than that of C-l, suggesting that the mechanical strength was enhanced upon dual crosslinking in this study also. The adhesion study indicates that silver ink adhesion on the gelation surface is better than that of carbon ink. In addition, the electrical response of C-2 with a screen-printed electrode (SPE) was found to be the same as the commercial polycarbonate (PC) substrate. The result of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay suggested that the silver SPE on C-2 was non-cytotoxic toward L929 fibroblast cells proliferation. The results indicated that C-2 gelatin is a promising material to act as a screen-printing substrate with excellent biodegradable and biocompatible properties.
本研究聚焦于可生物降解的天然及改性明胶膜作为丝网印刷基材的制备与评估。通过与各种交联剂交联制备改性明胶膜，并通过丝网印刷设计电极阵列。观察发现，用戊二醛和EDC/NHS（1-乙基-3-(3-二甲基氨基丙基)碳二亚胺/N-羟基琥珀酰亚胺）交联的C-2的溶胀率（3.98%）低于仅用戊二醛交联的C-1（8.77%）和未交联的C-0（28.15%）。所得结果表明，C-1和C-2的溶胀率均低于C-0（未交联的对照）。C-1和C-2的杨氏模量分别为8.55±0.57和23.72±2.04 kPa。因此，可以得出结论，C-2的机械强度是C-1的两倍，这表明在本研究中双重交联也提高了机械强度。附着力研究表明，银墨在凝胶表面的附着力优于碳墨。此外，发现C-2与丝网印刷电极（SPE）的电响应与商用聚碳酸酯（PC）基材相同。MTT（3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐）测定结果表明，C-2上的银SPE对L929成纤维细胞增殖无细胞毒性。结果表明，C-2明胶是一种有前途的材料，可作为具有优异生物可降解性和生物相容性的丝网印刷基材。
