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[利用抗胰蛋白酶沉淀血清鉴定葡萄球菌蛋白酶]

[Identification of proteases of staphylococci by means of an antitrypsin precipitating serum].

作者信息

Krivoshein Iu S, Protsenko V A, Karpitskiĭ V V, Spitsyn I F

出版信息

Biull Eksp Biol Med. 1975 Sep;80(9):59-61.

PMID:1222277
Abstract

Specific antitrypsin precipitating sera obtained by intraconjunctival immunization of rabbits with crystalline trypsin was used to reveal in the supernant of staphylococcal cultures protease having antigenic character common with that of trypsin. This is confirmed by the trypsin accumulation in the process of staphylococcus cultivation, by the inhibition of the proteolytic activity of the antitrypsin precipitating sera and by the production of protein-precipitate as a result of incubation of the supernant with the antitrypsin precipitating sera.

摘要

通过用结晶胰蛋白酶对兔进行结膜内免疫获得的特异性抗胰蛋白酶沉淀血清,用于揭示葡萄球菌培养物上清液中具有与胰蛋白酶共同抗原特性的蛋白酶。葡萄球菌培养过程中胰蛋白酶的积累、抗胰蛋白酶沉淀血清的蛋白水解活性受到抑制以及上清液与抗胰蛋白酶沉淀血清孵育产生蛋白质沉淀,均证实了这一点。

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