Axelrood Paige E, Chow Monica L, Arnold Clarke S, Lu Karen, McDermott Joseph M, Davies Julian
BC Research Inc, Vancouver, Canada.
Can J Microbiol. 2002 Jul;48(7):643-54. doi: 10.1139/w02-058.
Bacteria from forest surface organic matter and mineral soil horizons were cultivated using four methods and characterized by fatty acid methyl ester (FAME) analysis. Soil samples from a British Columbia Ministry of Forests Long-Term Soil Productivity (LTSP) installation were collected during winter and summer from two disturbance treatments (whole-tree harvesting with no soil compaction (plot N) and whole-tree harvesting plus complete surface organic matter removal with heavy soil compaction (plot S)) and from an unlogged reference plot (REF). Seventy-five percent of 1795 bacterial isolates were affiliated with 42 genera representing beta- and gamma-Proteobacteria, Actinobacteria, the Bacillus/Clostridium group, and the Cytophaga-Flexibacter-Bacteroides group. Approximately half of the culture collection represented genetic diversity confined to four bacterial genera: Pseudomonas, Bacillus, Paenibacillus, and Arthrobacter. A significantly higher proportion of bacterial isolates belonging to Actinobacteria, and the member genus Arthrobacter, were isolated from plot S soil samples compared with soil samples from plots N and REF. Twenty-five percent of bacterial isolates were not conclusively identified to genus with FAME analysis. Sherlock Tracker cluster analysis and partial 16S rRNA gene sequence analysis enabled classification of a subset of these isolates.
采用四种方法培养了来自森林表层有机质和矿质土壤层的细菌，并通过脂肪酸甲酯（FAME）分析对其进行了表征。在冬季和夏季，从不列颠哥伦比亚省森林部长期土壤生产力（LTSP）设施采集土壤样本，这些样本来自两种干扰处理（无土壤压实的全树采伐（地块N）和全树采伐加完全去除表层有机质并重度土壤压实（地块S））以及一个未采伐的参考地块（REF）。1795株细菌分离株中有75%隶属于42个属，这些属代表β-和γ-变形菌纲、放线菌、芽孢杆菌/梭菌组以及噬纤维菌-屈挠杆菌-拟杆菌组。大约一半的培养物集合代表了局限于四个细菌属的遗传多样性：假单胞菌属、芽孢杆菌属、类芽孢杆菌属和节杆菌属。与地块N和REF的土壤样本相比，从地块S的土壤样本中分离出的属于放线菌以及成员属节杆菌的细菌分离株比例显著更高。通过FAME分析，25%的细菌分离株无法最终确定到属。Sherlock Tracker聚类分析和部分16S rRNA基因序列分析能够对这些分离株的一个子集进行分类。
