Koekemoer L L, Kamau L, Hunt R H, Coetzee M
Department of Clinical Microbiology and Infectious Diseases, School of Pathology of the National Health Laboratory Services and the University of the Witwatersrand, Johannesburg, South Africa.
Am J Trop Med Hyg. 2002 Jun;66(6):804-11. doi: 10.4269/ajtmh.2002.66.804.
Anopheles funestus Giles is a major malaria vector in Africa belonging to a group of species with morphologically similar characteristics. Morphological identification of members of the A. funestus group is difficult because of overlap of distinguishing characteristics in adult or immature stages as well as the necessity to rear isofemale lines to examine larval and egg characters. A rapid rDNA polymerase chain reaction (PCR) method has been developed to accurately identify five members of the A. funestus group. This PCR is based on species-specific primers in the ITS2 region on the rDNA to identify A. funestus (approximately 505bp), Anopheles vaneedeni Gillies and Coetzee (approximately 587bp), Anopheles rivulorum Leeson (approximately 411bp), Anopheles leesoni Evans (approximately 146bp), and Anopheles parensis Gillies (approximately 252bp).
冈比亚按蚊是非洲主要的疟疾传播媒介，属于一组形态特征相似的物种。由于成虫或未成熟阶段鉴别特征的重叠，以及需要培育同雌系来检查幼虫和卵的特征，冈比亚按蚊组成员的形态学鉴定很困难。已开发出一种快速核糖体DNA聚合酶链反应（PCR）方法来准确鉴定冈比亚按蚊组的五个成员。该PCR基于核糖体DNA ITS2区域中的物种特异性引物，以鉴定冈比亚按蚊（约505bp）、瓦内登按蚊（约587bp）、溪流按蚊（约411bp）、利氏按蚊（约146bp）和帕伦西斯按蚊（约252bp）。
