Kuroki Keiichi, Kreeger John M, Cook James L, Tomlinson James L, Johnson Gayle C, Pace Lanny W, Turnquist Susan E, Turk James R, Ramos Jose A, Miller Margaret A
Comparative Orthopaedic Laboratory, College of Veterinary Medicine, University of Missouri, Columbia 65211, USA.
Am J Vet Res. 2002 Sep;63(9):1285-91. doi: 10.2460/ajvr.2002.63.1285.
To determine immunoreactivity of matrix metalloproteinase (MMP)-1, -3, and -13 in cartilaginous tumors of dogs, correlate expression of MMP with histologic grade of tumors and clinical outcome of dogs, and compare MMP immunoreactivity between chondrosarcomas and chondromas.
Formalin-fixed, paraffin-embedded tissues obtained from samples of naturally occurring chondrosarcomas (n = 31) and chondromas (8) of dogs that were submitted to our veterinary medical diagnostic laboratory.
Histologic sections from each sample were stained with H&E and monoclonal antibody to MMP-1, -3, and -13 by use of an avidin-peroxidase immunohistochemical technique. For each section, histologic grade (I, II, or III) and immunohistochemical expression (0, 1, 2, or 3) were evaluated. Clinical outcome was obtained from medical records or interviews with referring veterinarians and scored as a good outcome, moderate outcome, or poor outcome. Correlations among variables and differences between chondrosarcomas and chondromas were analyzed.
Samples from chondrosarcomas had significantly higher immunoreactivity of MMP-1 and -13, compared with immunoreactivity in samples from chondromas. In chondrosarcomas, a significant positive correlation (r, 0.386) was found between MMP-1 and -13 immunoreactivities, and a significant negative correlation (r, -0.390) was detected between MMP-3 and -13 immunoreactivities.
A significant increase in expression of collagenases (MMP-1 and -13) in chondrosarcomas, compared with expression in chondromas, suggests that collagenases may play an important role in tumor progression, and possibly metastasis, in chondrosarcomas of dogs.
确定基质金属蛋白酶（MMP）-1、-3和-13在犬软骨肿瘤中的免疫反应性，将MMP的表达与肿瘤的组织学分级及犬的临床结局相关联，并比较软骨肉瘤和软骨瘤之间的MMP免疫反应性。
取自提交至我们兽医医学诊断实验室的自然发生的犬软骨肉瘤（n = 31）和软骨瘤（8个）样本的福尔马林固定、石蜡包埋组织。
每个样本的组织学切片用苏木精和伊红（H&E）染色，并采用抗生物素蛋白-过氧化物酶免疫组织化学技术用抗MMP-1、-3和-13单克隆抗体染色。对每个切片评估组织学分级（I、II或III）和免疫组织化学表达（0、1、2或3）。从医疗记录或与转诊兽医的访谈中获取临床结局，并将其评为良好结局、中等结局或不良结局。分析变量之间的相关性以及软骨肉瘤和软骨瘤之间的差异。
与软骨瘤样本中的免疫反应性相比，软骨肉瘤样本中MMP-1和-13的免疫反应性显著更高。在软骨肉瘤中，MMP-1和-13免疫反应性之间存在显著正相关（r = 0.386），MMP-3和-13免疫反应性之间存在显著负相关（r = -0.390）。
与软骨瘤中的表达相比，软骨肉瘤中胶原酶（MMP-1和-13）表达的显著增加表明，胶原酶可能在犬软骨肉瘤的肿瘤进展及可能的转移中起重要作用。
