Kawashima A, Okada Y, Nakanishi I, Ueda Y, Iwata K, Roessner A
Department of Pathology, Faculty of Medicine, Otto-von-Guericke University, Magdeburg, Germany.
Gen Diagn Pathol. 1997 Feb;142(3-4):129-37.
We have immunohistochemically examined the localization of matrix metalloproteinases (MMPs) and tissue inhibitors of matrix metalloproteinases (TIMPs) in human chondrosarcomas (CHS) (23 cases) and benign chondroid lesions (BCL) (16 cases of osteochondromas and 11 cases of enchondromas). In CHS, all the MMPs and TIMPs examined were positive. Among them, MMP-1 was immunolocalized in more than 90% of both CHS and BCL, but positive score of MMP-1 was significantly higher in CHS than that in BCL (p < 0.01). Compared with BCL, CHS expressed MMP-3 at a low level, and more often positive in MMP-9. It is possible that chondrosarcoma might have a tendency to lose the ability to secrete MMP-3, which is a metalloproteinase that can degrade cartilage proteoglycans and is related to normal cartilage turnover. MMP-2, TIMP-1 and TIMP-2 were immunolocalized in more than 70% of the cases of both BCL and CHS, but the positive scores of these were not statistically different between the two groups. Interestingly, in several cases of CHS, both MMP-1 and MMP-9 immunostains were observed preferentially within the cells at the marginal areas of cartilaginous lobules. These findings suggest that increased expression of MMP-1 and MMP-9 and decrease in MMP-3 expression are associated with the malignant phenotype of the cartilaginour tumors.
我们采用免疫组织化学方法检测了基质金属蛋白酶（MMPs）和基质金属蛋白酶组织抑制剂（TIMPs）在23例人软骨肉瘤（CHS）以及16例骨软骨瘤和11例内生软骨瘤等良性软骨样病变（BCL）中的定位情况。在CHS中，所检测的所有MMPs和TIMPs均呈阳性。其中，MMP - 1在CHS和BCL中免疫定位均超过90%，但CHS中MMP - 1的阳性评分显著高于BCL（p < 0.01）。与BCL相比，CHS中MMP - 3表达水平较低，而MMP - 9阳性更为常见。软骨肉瘤可能有失去分泌MMP - 3能力的倾向，MMP - 3是一种能降解软骨蛋白聚糖且与正常软骨更新相关的金属蛋白酶。MMP - 2、TIMP - 1和TIMP - 2在BCL和CHS的70%以上病例中免疫定位，但两组间这些指标的阳性评分无统计学差异。有趣的是，在几例CHS中，在软骨小叶边缘区域的细胞内优先观察到MMP - 1和MMP - 9免疫染色。这些发现提示MMP - 1和MMP - 9表达增加以及MMP - 3表达降低与软骨肿瘤的恶性表型相关。
