Specific determination of estriol-16-glucuronide from the urine of pregnant women in the last trimester.

A method for quantitative estimation of estriol-16-glucuronide in urine of pregnant women is given. The glucuronide is isolated by precipitation with ammoniumsulfate (70% w/v) followed by extraction with ether-ethanol (3:1). The estriol-16-glucuronide is converted with dark blue r to an azodye, which is separated from colored impurities by passing through a small column of sodium sulfate. The absorbance of the eluted dye is measured at 520 nm. 75 cases were analyzed and it was found that the estriol-16-glucuronide excretion increases in the 3rd trimester of pregnancy from the average value of 9.06 mg/24 hours in the 28th week to 22.11 mg/24 hours in the 42nd week of pregnancy.