Mozsolits Henriette, Aguilar Marie-Isabel
Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, 3800, Australia.
Biopolymers. 2002;66(1):3-18. doi: 10.1002/bip.10200.
The interactions between peptides and membranes mediate a wide variety of biological processes, and characterization of the molecular details of these interactions is central to our understanding of cellular events such as protein trafficking, cellular signaling and ion-channel formation. A wide variety of biophysical techniques have been combined with the use of model membrane systems to study peptide-membrane interactions, and have provided important information on the relationship between membrane-active peptide structure and their biological function. However, what has generally not been reported is a detailed analysis of the affinity of peptide for different membrane systems, which has largely been due to the difficulty in obtaining this information. To address this issue, surface plasmon resonance (SPR) spectroscopy has recently been applied to the study of biomembrane-based systems using both planar mono- or bilayers or liposomes. This article provides an overview of these recent applications that demonstrate the potential of SPR to enhance our molecular understanding of membrane-mediated peptide function.
肽与膜之间的相互作用介导了多种生物学过程，对这些相互作用的分子细节进行表征是我们理解诸如蛋白质运输、细胞信号传导和离子通道形成等细胞事件的核心。多种生物物理技术已与模型膜系统结合使用，以研究肽与膜的相互作用，并提供了有关膜活性肽结构与其生物学功能之间关系的重要信息。然而，尚未普遍报道的是对肽与不同膜系统亲和力的详细分析，这主要是由于获取此类信息存在困难。为了解决这个问题，表面等离子体共振（SPR）光谱最近已应用于使用平面单层或双层膜或脂质体的基于生物膜的系统的研究。本文概述了这些最新应用，这些应用展示了SPR在增强我们对膜介导的肽功能的分子理解方面的潜力。
