Payne Jennifer A E, HsienLee Tzong, Anderson Marilyn A, Aguilar Marie-Isabel
Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Victoria, Australia.
Monash Biomedicine Discovery Institute and Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, Australia.
Bio Protoc. 2017 Jan 5;7(1):e2087. doi: 10.21769/BioProtoc.2087.
Examining the interaction of peptides with lipid bilayers to determine binding kinetics is often performed using surface plasmon resonance (SPR). Here we describe the technique of dual polarisation interferometry (DPI) that provides not only information on the kinetics of the peptide binding to the bilayer, but also how the peptide affects the lipid order of the bilayer.
研究肽与脂质双层的相互作用以确定结合动力学通常使用表面等离子体共振(SPR)来进行。在此,我们描述了双偏振干涉测量法(DPI)技术,该技术不仅能提供肽与双层结合动力学的信息,还能提供肽如何影响双层脂质有序性的信息。