Gold Barry
Eppley Institute for Research in Cancer and Department of Pharmaceutical Sciences, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, NE 68198-6805, USA.
Biopolymers. 2002 Nov 5;65(3):173-9. doi: 10.1002/bip.10215.
DNA is normally considered to be a stiff rod-like molecule, but proteins and small molecules, which either equilibrium bind to, or covalent bond with, DNA, can overcome the barrier(s) to non-linearity by changing the local hydrophobic and electrostatic environment at specific DNA sequences. The deformation of DNA by proteins has been shown to be a critical event in transcriptional regulation. In this review, we have focused on how the introduction of cationic charge, and its location, can affect DNA structure. To study the effect of charge, we have used DNA modified with 3-aminopropyl substitutions at the 5-position of deoxyuracil that mimic basic lysine-like sidechains, and which place the cationic charge in the major groove. Previous gel mobility studies with these sidechains have shown that they bend DNA. The location of the cationic sidechains and how they bend DNA is discussed.
DNA通常被认为是一种刚性的棒状分子，但与DNA平衡结合或共价结合的蛋白质和小分子，可以通过改变特定DNA序列处的局部疏水和静电环境来克服非线性障碍。蛋白质对DNA的变形已被证明是转录调控中的关键事件。在本综述中，我们重点关注阳离子电荷的引入及其位置如何影响DNA结构。为了研究电荷的影响，我们使用了在脱氧尿嘧啶5位用3-氨丙基取代修饰的DNA，这些取代模拟碱性赖氨酸样侧链，并将阳离子电荷置于大沟中。先前对这些侧链进行的凝胶迁移率研究表明，它们会使DNA弯曲。本文讨论了阳离子侧链的位置及其使DNA弯曲的方式。
