甘露碱合成酶启动子调控中衰老成分的证明。
Demonstration of a Senescence Component in the Regulation of the Mannopine Synthase Promoter.
作者信息
Ursin V. M., Shewmaker C. K.
机构信息
Calgene Inc., 1920 Fifth Street, Davis, California 95616.
出版信息
Plant Physiol. 1993 Jul;102(3):1033-1036. doi: 10.1104/pp.102.3.1033.
Abstract
Regulation of the mannopine synthase (mas) promoter during senescence in leaves and flowers of tobacco (Nicotiana tabacum) plants was investigated. In plants transformed with a mas 5[prime]-[beta]-glucuronidase (GUS)-mas 3[prime] transcriptional fusion, we observed that following the onset of senescence in either intact or excised leaves of the transgenic lines, GUS activity increased significantly, whereas in excised leaves in which the senescence process was inhibited, GUS activity increased only marginally. During flower petal senescence in the transgenic tobacco, GUS activity increased approximately 6-fold over preanthesis- and anthesis-stage flowers.
摘要
研究了烟草（Nicotiana tabacum）植株叶片和花朵衰老过程中甘露碱合成酶（mas）启动子的调控。在用mas 5′-β-葡萄糖醛酸酶（GUS）-mas 3′转录融合体转化的植株中，我们观察到，在转基因系完整或离体叶片衰老开始后，GUS活性显著增加，而在衰老过程受到抑制的离体叶片中，GUS活性仅略有增加。在转基因烟草花瓣衰老过程中，GUS活性比花前和花期花朵增加了约6倍。
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