An G, Costa M A, Ha S B
Institute of Biological Chemistry, Washington State University, Pullman 99164-6340.
Plant Cell. 1990 Mar;2(3):225-33. doi: 10.1105/tpc.2.3.225.
The activity of the nopaline synthase (nos) promoter is differentially regulated in several plant organs. In this article we demonstrate that the nos promoter is wound inducible in both vegetative and reproductive organs. The induction of the nos promoter was observed in leaves, stems, cotyledons, and various reproductive organs, suggesting that the response is not organ specific. The wound response was further enhanced by addition of auxins. Other growth substances had no effect on the wound-inducible nos promoter activity. Deletion analysis of the nos promoter indicated that the 10-base pair (GCACATACGT) Z element located between -123 and -114 or an element overlapping with this sequence is essential for the wound and auxin responses.
胭脂碱合成酶(nos)启动子的活性在几种植物器官中受到不同的调控。在本文中,我们证明了nos启动子在营养器官和生殖器官中均受创伤诱导。在叶片、茎、子叶和各种生殖器官中均观察到nos启动子的诱导,这表明该反应并非器官特异性的。添加生长素可进一步增强创伤反应。其他生长物质对创伤诱导的nos启动子活性没有影响。对nos启动子的缺失分析表明,位于-123至-114之间的10个碱基对(GCACATACGT)Z元件或与该序列重叠的元件对于创伤和生长素反应至关重要。