University of Alberta, Plant Molecular Genetics and Plant Biotechnology Centre and Department of Cell Biology, Medical Sciences Building, Edmonton, AB T6G 2P5, Canada.
Proc Natl Acad Sci U S A. 1989 May;86(9):3219-23. doi: 10.1073/pnas.86.9.3219.
Temporal and spacial distribution of mannopine synthase (mas) promoter activity was determined throughout the development of transgenic tobacco plants using bacterial luciferase luxA and luxB as reporter genes. Luciferase activity was determined by luminometry in vitro and visualized by computer-enhanced single-photon video imaging in vivo. The activity of the mas dual promoters increased basipetally in developing plants and was wound-inducible in leaf and stem tissue. Hormone bioassays with isolated plant tissues and tumors deficient in the transferred DNA (T-DNA)-encoded genes iaaM, iaaH, and ipt indicated that activity of the mas dual promoters is regulated by auxin and enhanced by cytokinin in both differentiated and tumorous plant cells.
采用细菌荧光素酶 luxA 和 luxB 作为报告基因，通过活体单光子视频成像技术，在体外通过发光测定法，对转基因烟草植株发育过程中 mannopine 合酶（mas）启动子活性的时空分布进行了测定。荧光素酶活性在发育中的植株中沿基端向上增加，并在叶片和茎组织中受伤诱导。对离体植物组织和缺失转移 DNA（T-DNA）编码基因 iaaM、iaaH 和 ipt 的肿瘤进行激素生物测定表明，mas 双启动子的活性受生长素的调控，并在分化和肿瘤植物细胞中受细胞分裂素的增强。
