激发子隐地蛋白可诱导烟草细胞中的蛋白质磷酸化。
Protein Phosphorylation Is Induced in Tobacco Cells by the Elicitor Cryptogein.
作者信息
Viard M. P., Martin F., Pugin A., Ricci P., Blein J. P.
机构信息
Institut National de la Recherche Agronomique, Universite de Bourgogne, Laboratoire de Phytopharmacie Phytobiologie Cellulaire, BV 1540 21034 Dijon Cedex, France (M.-P.V., F.M., A.P.J.-P.B.).
出版信息
Plant Physiol. 1994 Apr;104(4):1245-1249. doi: 10.1104/pp.104.4.1245.
Changes in plasmalemma ion fluxes were observed when tobacco (Nicotiana tabacum) cells were treated with cryptogein, a proteinaceous elicitor from Phytophthora cryptogea. A strong alkalization of the culture medium, accompanied by a leakage of potassium, was induced within a few minutes of treatment. These effects reached a maximum after 30 to 40 min and lasted for several hours. This treatment also resulted in a rapid, but transient, production of activated oxygen species. All these physiological responses were fully sensitive to staurosporine, a known protein kinase inhibitor. Furthermore, a study of protein phosphorylation showed that cryptogein induced a staurosporine-sensitive phosphorylation of several polypeptides. These data suggest that phosphorylated proteins may be essential for the transduction of elicitor signals.
当烟草（Nicotiana tabacum）细胞用隐地蛋白（一种来自隐地疫霉的蛋白质激发子）处理时，观察到质膜离子通量的变化。处理后几分钟内，培养基会强烈碱化，并伴有钾离子泄漏。这些效应在30至40分钟后达到最大值，并持续数小时。这种处理还导致活性氧的快速但短暂的产生。所有这些生理反应对已知的蛋白激酶抑制剂星形孢菌素完全敏感。此外，蛋白质磷酸化研究表明，隐地蛋白诱导了几种多肽的星形孢菌素敏感的磷酸化。这些数据表明，磷酸化蛋白可能是激发子信号转导所必需的。
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