North China Key Laboratory for Germplasm Resources of Education Ministry, Department of Agriculture, Hebei Agricultural University, Baoding, 071001, People's Republic of China.
Plant Cell Rep. 2013 Nov;32(11):1703-13. doi: 10.1007/s00299-013-1481-7. Epub 2013 Aug 4.
Overexpression of a cotton defense-related gene GbSTK in Arabidopsis resulted in enhancing pathogen infection and oxidative stress by activating multiple defense-signaling pathways.
Serine/threonine protein kinase (STK) plays an important role in the plant stress-signaling transduction pathway via phosphorylation. Most studies about STK genes have been conducted with model species. However, their molecular and biochemical characterizations have not been thoroughly investigated in cotton. Here, we focused on one such member, GbSTK. RT-PCR indicated that it is induced not only by Verticillium dahliae Kleb., but also by signaling molecules. Subcellular localization showed that GbSTK is present in the cell membrane, cytoplasm, and nucleus. Overexpression of GbSTK in Arabidopsis resulted into the enhanced resistance to V. dahliae. Moreover, Overexpression of GbSTK elevated the expression of PR4, PR5, and EREBP, conferring on transgenic plants enhanced reactive oxygen species scavenging capacity and oxidative stress tolerance. Our results suggest that GbSTK is active in multiple defense-signaling pathways, including those involved in responses to pathogen infection and oxidative stress.
在拟南芥中过表达棉花防御相关基因 GbSTK 通过激活多条防御信号通路来增强病原菌感染和氧化应激。
丝氨酸/苏氨酸蛋白激酶（STK）通过磷酸化在植物应激信号转导途径中发挥重要作用。大多数关于 STK 基因的研究都是在模式物种中进行的。然而，它们在棉花中的分子和生化特性尚未得到彻底研究。在这里，我们专注于一个这样的成员，GbSTK。RT-PCR 表明，它不仅受到黄萎病菌的诱导，还受到信号分子的诱导。亚细胞定位表明 GbSTK 存在于细胞膜、细胞质和细胞核中。在拟南芥中过表达 GbSTK 导致对黄萎病菌的抗性增强。此外，过表达 GbSTK 提高了 PR4、PR5 和 EREBP 的表达，赋予转基因植物增强的活性氧清除能力和氧化应激耐受性。我们的结果表明，GbSTK 活跃于多条防御信号通路，包括参与对病原菌感染和氧化应激的反应。
