Cutting Brian, Tolman Joel R, Nanchen Steve, Bodenhausen Geoffrey
Institut de Chimie Moléculaire et Biologique, Ecole Polytechnique Fédérale de Lausanne, Switzerland.
J Biomol NMR. 2002 Jul;23(3):195-200. doi: 10.1023/a:1019800511340.
The determination of residual dipolar couplings (RDCs) by quantitative J spectroscopy methods such as Heteronuclear Single Quantum Correlation with Phase Encoded Coupling (HSQC-PEC) is prone to systematic errors that may be caused by differential attenuation during the conversion of orthogonal density operator components into observable terms. The attenuation may be caused by miscalibration of radio-frequency pulses and by relaxation effects. A simple method is presented that allows one to remove most of these systematic errors without losses in sensitivity or resolution.
通过定量J光谱方法（如带有相位编码耦合的异核单量子相关谱，HSQC - PEC）来测定剩余偶极耦合（RDC）容易产生系统误差，这些误差可能是由于在将正交密度算符分量转换为可观测项的过程中存在差异衰减所致。这种衰减可能是由射频脉冲校准错误以及弛豫效应引起的。本文提出了一种简单的方法，该方法能够去除大部分此类系统误差，同时不会损失灵敏度或分辨率。
