在表现出核基因沉默的转基因植物中病毒积累的抑制
Suppression of Virus Accumulation in Transgenic Plants Exhibiting Silencing of Nuclear Genes.
作者信息
English J. J., Mueller E., Baulcombe D. C.
机构信息
The Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich, NR4 7UH, United Kingdom.
出版信息
Plant Cell. 1996 Feb;8(2):179-188. doi: 10.1105/tpc.8.2.179.
Abstract
Homology-dependent gene silencing contributes to variation between transgenic plants with respect to transgene and/or endogenous gene expression levels. Recent studies have linked post-transcriptional gene silencing and virus resistance in plants expressing virus-derived transgenes. Using a potato virus X vector, we present three examples in which silencing of nonviral transgenes prevented virus accumulation. This effect was dependent on sequence homology between the virus and the silenced transgene. Analysis of potato virus X derivatives carrying bacterial [beta]-glucuronidase (GUS) sequences showed that the 3[prime] region of the GUS coding sequence was a target of the silencing mechanism in two independent tobacco lines. Methylation of the silenced GUS transgenes in these lines was also concentrated in the 3[prime] region of the GUS coding sequence. Based on this concurrence, we propose a link between the DNA-based transgene methylation and the RNA-based gene silencing process.
摘要
同源依赖性基因沉默导致转基因植物在转基因和/或内源基因表达水平上存在差异。最近的研究将植物中表达病毒衍生转基因时的转录后基因沉默与病毒抗性联系起来。利用马铃薯X病毒载体，我们给出了三个例子，其中非病毒转基因的沉默阻止了病毒积累。这种效应取决于病毒与沉默转基因之间的序列同源性。对携带细菌β-葡萄糖醛酸酶（GUS）序列的马铃薯X病毒衍生物的分析表明，GUS编码序列的3′区域是两个独立烟草品系中沉默机制的靶点。这些品系中沉默的GUS转基因的甲基化也集中在GUS编码序列的3′区域。基于这种一致性，我们提出了基于DNA的转基因甲基化与基于RNA的基因沉默过程之间的联系。
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