Dimcheva Nina, Horozova Elena, Jordanova Zinaida
Department of Physical Chemistry, University of Plovdiv, Bulgaria.
Z Naturforsch C J Biosci. 2002 Jul-Aug;57(7-8):705-11. doi: 10.1515/znc-2002-7-826.
Glucose oxidase (E. C. 1.1.3.4) was immobilized on electrochemically modified graphite to obtain an enzyme electrode. The working surface of the electrode was coated with gelatine to prevent desorption of the enzyme. In substrate (glucose) solutions the amperometric signal of the enzyme electrode was due to the electroreduction of H202 generated in the enzyme layer. The linearity of the electrode response was found up to a substrate concentration of 300 microM at a working potential of 0 mV (vs. Ag/AgCl). It was shown that the electrode did not respond to L-ascorbic and uric acid at that working potential. The response time was about 2 min. The enzyme electrode keeps about 50% of its initial activity after a one-week storage at 4 degrees C.
葡萄糖氧化酶（E.C. 1.1.3.4）被固定在经电化学修饰的石墨上，以获得一种酶电极。电极的工作表面涂有明胶，以防止酶的解吸。在底物（葡萄糖）溶液中，酶电极的安培信号归因于酶层中产生的H2O2的电还原。在0 mV（相对于Ag/AgCl）的工作电位下，发现电极响应在高达300 microM的底物浓度范围内呈线性。结果表明，在该工作电位下，电极对L-抗坏血酸和尿酸无响应。响应时间约为2分钟。在4℃下储存一周后，酶电极保留了其初始活性的约50%。
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