Department of Applied Sciences, University of the West of England, Coldharbour Lane, Bristol BS16 1QY, UK.
Talanta. 2012 Sep 15;99:989-96. doi: 10.1016/j.talanta.2012.07.082. Epub 2012 Aug 4.
Silver paste electrodes modified with lyotropic phases formed from dodecyl benzenesulphonic acid and KCl were used as the reductant in the determination of the hydrogen peroxide released from the enzymatic reaction of glucose oxidase with glucose and oxygen. The response of the modified electrode to hydrogen peroxide reduction (-0.1 V vs. Ag/AgCl) was shown to suffer from interference resulting from co-localization of enzyme and substrate at the electrode surface. This interference was eradicated by the introduction of a perm-selective membrane in the form of cellulose acetate. This further facilitated immobilization of the enzyme while allowing diffusion of the generated peroxide to the electrode. The resulting configuration was shown to be capable of the analytical determination of glucose.
银膏电极用十二烷基苯磺酸和 KCl 形成的溶致相修饰，用作葡萄糖氧化酶与葡萄糖和氧气的酶促反应释放的过氧化氢的还原剂。修饰电极对过氧化氢还原（相对于 Ag/AgCl 为-0.1V）的响应被证明受到干扰，原因是酶和底物在电极表面的共定位。通过在醋酸纤维素的形式中引入一种具有选择性的渗透膜，消除了这种干扰。这进一步促进了酶的固定化，同时允许生成的过氧化物扩散到电极。结果表明，所得到的配置能够分析测定葡萄糖。
