Haque Sufia Ul, Naushad Mu
Department of Applied Chemistry, Faculty of Engineering and Technology, Aligarh Muslim University, Aligarh 202002, India.
Department of Applied Chemistry, Faculty of Engineering and Technology, Aligarh Muslim University, Aligarh 202002, India.
Enzyme Microb Technol. 2016 Jun;87-88:29-36. doi: 10.1016/j.enzmictec.2016.02.011. Epub 2016 Feb 24.
In this study, a bioanode was developed by using layer-by-layer (LBL) assembly of sulfonated graphene (SG)/ferritin (Frt)/glucose oxidase (GOx). The SG/Frt biocomposite was used as an electron transfer elevator and mediator, respectively. Glucose oxidase (GOx) from Aspergillus niger was applied as a glucose oxidation biocatalyst. The electrocatalytic oxidation of glucose using GOx modified electrode increases with an increase in the concentration of glucose in the range of 10-50mM. The electrochemical measurements of the electrode was carried out by using cyclic voltammetry (CV) at different scan rates (20-100mVs(-1)) in 30mM of glucose solution prepared in 0.3M potassium ferrocyanide (K4Fe(CN)6) and linear sweep voltammetry (LSV). A saturation current density of 50±2mAcm(-2) at a scan rate of 100mVs(-1) for the oxidation of 30Mm glucose is achieved.
在本研究中，通过磺化石墨烯（SG）/铁蛋白（Frt）/葡萄糖氧化酶（GOx）的层层（LBL）组装制备了一种生物阳极。SG/Frt生物复合材料分别用作电子传递提升器和媒介体。来源于黑曲霉的葡萄糖氧化酶（GOx）用作葡萄糖氧化生物催化剂。使用GOx修饰电极对葡萄糖的电催化氧化作用在10 - 50mM葡萄糖浓度范围内随葡萄糖浓度的增加而增强。通过在0.3M亚铁氰化钾（K4Fe(CN)6）中制备的30mM葡萄糖溶液中以不同扫描速率（20 - 100mVs(-1)）使用循环伏安法（CV）和线性扫描伏安法（LSV）对电极进行电化学测量。在100mVs(-1)的扫描速率下，对于30mM葡萄糖氧化实现了50±2mAcm(-2)的饱和电流密度。
