El Tayeb Amna B, Hanson Robert P
Department of Veterinary Preventive Medicine, The Ohio State University, Columbus 43210, USA.
Avian Dis. 2002 Jul-Sep;46(3):660-7. doi: 10.1637/0005-2086(2002)046[0660:IBECAN]2.0.CO;2.
We investigated the interaction between Newcastle disease virus (NDV) and Escherichia coli in cell cultures, embryonated eggs, and 8-wk-old chickens. We measured the interactions on the basis of bacterial adherence and NDV hemagglutination titer in chickens, chicken embryos, and chicken embryo cell culture. Depending on the inoculation order of E. coli, a significant alteration of the growth of NDV was observed in both chickens and chicken embryos. When certain strains of E. coli were given before NDV exposure, the virus titers were lowered. In chickens, the mean virus titer was significantly (P < 0.05) lowered in the crop, the proventriculus, the gizzard, and the jejunum. However, there were no significant differences (P < 0.05) between the two groups for NDV titers in the duodenum, ileum, and cecum. In chicken embryos, when E. coli serotypes O78 and O119:B14 were inoculated before NDV exposure, the mean NDV titers were significantly (P < 0.5) lowered. However, there were no significant differences (P < 0.05) in NDV titer between the two groups when E. coli serotypes O78:K80:NM and O1ab:K NM were inoculated 24 hr before NDV exposure. When NDV was given prior to E. coli exposure, NDV titer was higher in both chickens and chicken embryos. In chickens, when NDV was given 48 hr before E. coli inoculation, NDV was detected in the proventriculus, gizzard, jejunum, ileum, and cecum, whereas no virus was detected in the control groups (NDV only). In the crop, NDV was detected at a significantly (P < 0.05) higher titer in the E. coli-inoculated group when compared with the control group that received NDV alone. In chicken embryos, virus titer was significantly (P < 0.05) higher when NDV was given 24 hr before E. coli inoculation for all three NDV strains used (Ulster and V4 strains). Adherence of E. coli to chicken embryo kidney (CEK) cells was significantly higher (P < 0.05) when the CEK cells were infected first with NDV and then by E. coli. The mean bacterial count per microscopic field in NDV-uninfected monolayers was eight compared with 112 for the NDV-infected monolayers. In approximately 10% of the fields in NDV-infected monolayers, the bacteria were too numerous to count.
我们研究了新城疫病毒（NDV）与大肠杆菌在细胞培养物、鸡胚和8周龄鸡中的相互作用。我们根据鸡、鸡胚和鸡胚细胞培养物中的细菌黏附情况以及NDV血凝效价来衡量这种相互作用。根据大肠杆菌的接种顺序，在鸡和鸡胚中均观察到NDV生长的显著变化。当在接触NDV之前给予某些大肠杆菌菌株时，病毒滴度会降低。在鸡中，嗉囊、腺胃、肌胃和空肠中的平均病毒滴度显著降低（P < 0.05）。然而，十二指肠、回肠和盲肠中两组的NDV滴度之间没有显著差异（P < 0.05）。在鸡胚中，当在接触NDV之前接种大肠杆菌血清型O78和O119:B14时，平均NDV滴度显著降低（P < 0.5）。然而，当在接触NDV前24小时接种大肠杆菌血清型O78:K80:NM和O1ab:K NM时，两组之间的NDV滴度没有显著差异（P < 0.05）。当在接触大肠杆菌之前给予NDV时，鸡和鸡胚中的NDV滴度均较高。在鸡中，当在接种大肠杆菌前48小时给予NDV时，在腺胃、肌胃、空肠、回肠和盲肠中检测到了NDV，但在对照组（仅接种NDV）中未检测到病毒。在嗉囊中，与仅接种NDV的对照组相比，接种大肠杆菌组的NDV滴度显著更高（P < 0.05）。在鸡胚中，对于所有三种使用的NDV毒株（阿尔斯特和V4毒株），当在接种大肠杆菌前24小时给予NDV时，病毒滴度显著更高（P < 0.05）。当鸡胚肾（CEK）细胞先感染NDV然后再感染大肠杆菌时，大肠杆菌对CEK细胞的黏附显著更高（P < 0.05）。未感染NDV的单层细胞中每个显微镜视野的平均细菌数为8个，而感染NDV的单层细胞中为112个。在感染NDV的单层细胞中，约10%的视野中细菌数量太多无法计数。
