Kijewska Agnieszka, Rokicki Jerzy, Sitko Jiri, Wegrzyn Grzegorz
Department of Invertebrate Zoology, University of Gdańsk, Pilz.shtsls;sudskiego 46, Gdynia 81-378, Poland.
Exp Parasitol. 2002 May;101(1):35-9. doi: 10.1016/s0014-4894(02)00031-0.
Eleven species belonging to superfamily Ascaridoidea, which infect marine and freshwater fish, mammals, and fish-eating birds, were analyzed using a PCR-RFLP method. The following species were investigated: Anisakis pegreffi, A. physeteris, and A. simplex (parasites of fish and mammals), Contracaecum osculatum, C. radiatum, and C. rudolphi (parasites of mammals and fish-eating birds), Hysterothylacium aduncum (a parasite of fish), Porrocaecum angusticolle, P. crassum, P. depressum, and P. ensicaudatum (parasites of fish-eating birds). PCR-amplified rDNA regions encompassing ITS1, 5.8S rDNA, and ITS2 produced on templates of genomic DNA isolated from all investigated species were digested with TaqI, AluI, BsuRI, and RsaI endonucleases. Restriction patterns showed that endonuclease TaqI is the most useful enzyme for identification of all investigated species. No variations in restriction patterns within each species were detected. Therefore, we propose that the PCR-RFLP assay described in this report may be used for identification of marine and freshwater parasites from superfamily Ascaridoidea.
使用PCR-RFLP方法分析了属于蛔总科的11个物种，这些物种感染海洋和淡水鱼类、哺乳动物以及食鱼鸟类。研究的物种如下：佩氏异尖线虫、抹香鲸异尖线虫和简单异尖线虫（鱼类和哺乳动物的寄生虫）、吻状对盲囊线虫、辐射对盲囊线虫和鲁氏对盲囊线虫（哺乳动物和食鱼鸟类的寄生虫）、钩状异唇线虫（鱼类的寄生虫）、窄管前盲囊线虫、粗管前盲囊线虫、压低前盲囊线虫和尾前盲囊线虫（食鱼鸟类的寄生虫）。用TaqI、AluI、BsuRI和RsaI内切酶消化从所有研究物种中分离的基因组DNA模板上PCR扩增的包含ITS1、5.8S rDNA和ITS2的rDNA区域。限制性图谱表明，内切酶TaqI是鉴定所有研究物种最有用的酶。未检测到每个物种内限制性图谱的变化。因此，我们建议本报告中描述的PCR-RFLP分析可用于鉴定蛔总科的海洋和淡水寄生虫。
