Waikagul Jitra, Dekumyoy Paron, Chaichana Kitiyaporn, Thairungroje Anantapruti Malinee, Komalamisra Chalit, Kitikoon Viroj
Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand.
Parasitol Int. 2002 Sep;51(3):237-47. doi: 10.1016/s1383-5769(02)00013-2.
Cocktail and electroeluted antigens from Bithynia goniomphalos, the snail intermediate host of Opisthorchis viverrini, were extracted and purified. The performance of these two antigens in the antibody detection of human opisthorchiasis was evaluated by indirect ELISA. Serum samples from people whose stool was either: (i). positive for Opisthorchis eggs (n=61); or (ii). positive for at least one of 19 other species of parasite (n=125); or (iii). clear of parasites (n=30) were tested. The sensitivity, specificity, positive predictive value and negative predictive value of ELISA using cocktail antigen were 88.5, 88, 78.2 and 94%, respectively; those of ELISA using eluted antigen (53 kDa) were 91.8, 98.4, 96.5 and 96.1%, respectively. Cross-reaction with the eluted antigen was seen in only one of four cases of hymenolepiasis and only one of 10 cases of strongyloidiasis. The kappa coefficients for ELISA in relation to stool examination were 0.84 (cocktail antigen) and 0.87 (eluted antigen). This study showed that Bithynia snail antigen could be used to replace worm antigen in the antibody detection of human O. viverrini infection.
从麝猫后睾吸虫的中间宿主——具瘤毕吸虫中提取并纯化了混合抗原和电洗脱抗原。通过间接ELISA评估了这两种抗原在人体华支睾吸虫病抗体检测中的性能。检测了粪便样本来自以下人群的血清：（i）华支睾吸虫卵阳性者（n = 61）；（ii）其他19种寄生虫中至少一种阳性者（n = 125）；或（iii）未感染寄生虫者（n = 30）。使用混合抗原的ELISA的敏感性、特异性、阳性预测值和阴性预测值分别为88.5%、88%、78.2%和94%；使用洗脱抗原（53 kDa）的ELISA的相应指标分别为91.8%、98.4%、96.5%和96.1%。在4例膜壳绦虫病患者中仅有1例与洗脱抗原发生交叉反应，在10例粪类圆线虫病患者中也仅有1例发生交叉反应。ELISA相对于粪便检查的kappa系数分别为0.84（混合抗原）和0.87（洗脱抗原）。本研究表明，在人体麝猫后睾吸虫感染的抗体检测中，具瘤毕吸虫抗原可用于替代虫体抗原。
