Watthanakulpanich D, Waikagul J, Anantaphruti M T, Dekumyoy P
Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Southeast Asian J Trop Med Public Health. 1997 Sep;28(3):593-8.
Four batches of crude somatic antigens from: (1) Opisthorchis viverrini adult worms, (2) Bithynia funiculata-whole body, (3) B. funiculata-head-foot, and (4) B. funiculata-visceral mass were assayed against sera from 81 opisthorchiasis patients, 30 parasite-free healthy individuals, and 50 individuals infected with other helminthic infections, and their antibody levels determined. By IgG-ELISA, the antigenic reactive proteins were found in both the head-foot and the visceral mass of B. funiculata snails, but the whole snail antigens gave the best results. Furthermore, it was as good as when O. viverini antigens were used. Antibody levels of sera from patients with opisthorchiasis assayed against antigens from whole B. funiculata snails were significantly higher than those of the other two groups. The cut-off value for positivity at 0.228 gave 80.2% sensitivity and 81.2% specificity. Cross reactions were observed with sera from patients with paragonimiasis and strongyloidiasis. No cross reactions were found to occur with sera from healthy individuals.
(1) 华支睾吸虫成虫；(2) 微小双脐螺全虫体；(3) 微小双脐螺头足部；(4) 微小双脐螺内脏团，检测对象为81名华支睾吸虫病患者、30名未感染寄生虫的健康个体以及50名感染其他蠕虫的个体的血清，并测定了他们的抗体水平。通过IgG-ELISA法发现，微小双脐螺的头足部和内脏团中均存在抗原反应性蛋白，但全螺抗原检测效果最佳。此外，其效果与使用华支睾吸虫抗原时相当。华支睾吸虫病患者血清针对微小双脐螺全虫体抗原的抗体水平显著高于其他两组。阳性临界值为0.228时，灵敏度为80.2%，特异性为81.2%。在并殖吸虫病和类圆线虫病患者的血清中观察到了交叉反应。在健康个体的血清中未发现交叉反应。
