Brown R. C., Lemmon B. E., Olsen O. A.
Department of Biology, University of Southwestern Louisiana, Lafayette, Louisiana 70504-2451.
Plant Cell. 1994 Sep;6(9):1241-1252. doi: 10.1105/tpc.6.9.1241.
An immunofluorescence study of sectioned barley endosperm imaged by confocal laser scanning microscopy provided three-dimensional data on the relationship of microtubules to the cytoplasm, nuclei, and cell walls during development from 4 to 21 days after pollination (DAP). Microtubules play an important role throughout endosperm ontogeny. The syncytium is organized into units of nuclear-cytoplasmic domains by nuclear-based radial microtubule systems that appear to control the pattern of the first anticlinal walls at 5 to 6 DAP. After 7 DAP, phragmoplasts of two origins (interzonal and cytoplasmic) guide wall formation. Large compartments formed by the "free growing" walls in association with cytoplasmic phragmoplasts formed adventitiously at interfaces of opposing microtubule systems are subsequently subdivided by interzonal phragmoplast/cell plates to give rise to the starchy endosperm. During development of the aleurone layer from 8 to 21 DAP, the microtubule cycle is typical of plant histogenesis; cortical microtubules are hooplike, and preprophase bands of microtubules predict the division plane.
通过共聚焦激光扫描显微镜对大麦胚乳切片进行的免疫荧光研究，提供了授粉后4至21天（DAP）发育过程中微管与细胞质、细胞核和细胞壁关系的三维数据。微管在胚乳个体发育过程中发挥着重要作用。合胞体通过基于核的径向微管系统组织成核质域单元，这些系统似乎在授粉后5至6天控制着第一道垂周壁的模式。授粉后7天，两种来源（区域间和细胞质）的成膜体引导细胞壁形成。由“自由生长”壁与在相对微管系统界面偶然形成的细胞质成膜体相关联形成的大隔室，随后被区域间成膜体/细胞板细分，从而产生淀粉胚乳。在糊粉层从授粉后8天到21天的发育过程中，微管循环是植物组织发生的典型特征；皮层微管呈环状，微管的前期带预测分裂平面。
