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[用乙酸对硝基苯酯测定人血清中的芳基酯酶活性]

[Determination of aryl esterase activity in the human serum using 4-nitrophenyl acetate].

作者信息

Koch C D, Block H, Molz J

出版信息

Z Gastroenterol. 1975 Dec;13(8):695-703.

PMID:1224758
Abstract

A new determination of esterases and arylesterases (E. C. 3.1.1.2.) with 4-nitrophenylacetate is described. The results are compared with those of the method described by Pilz using phenylacetate. 4-nitrophenylacetate instead of phenylacetate allows a kinetic reaction. The precision of the determination is good, the method easy to handle. Normal values: 1100--2600 U/1 (total esterases), 700--2100 U/1 (arylesterases) at +25 degrees C.

摘要

本文描述了一种用4-硝基苯乙酸酯对酯酶和芳基酯酶(E.C.3.1.1.2.)进行新的测定方法。将结果与皮尔兹使用苯乙酸酯所描述的方法的结果进行了比较。用4-硝基苯乙酸酯代替苯乙酸酯可实现动力学反应。该测定方法精度良好,易于操作。正常数值:在25摄氏度时,总酯酶为1100 - 2600 U/1,芳基酯酶为700 - 2100 U/1。

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