Wagle S R, Ingebretsen W R, Sampson L
Acta Diabetol Lat. 1975 May-Aug;12(3-4):185-98. doi: 10.1007/BF02581299.
Hepatocytes were isolated by collagenase in vitro perfusion technique. Net glucose production in isolated hepatocytes obtained from fed, fasted and alloxan diabetic rats was studied. Net glucose production from alanine, pyruvate and fructose was increased by 2-5 fold in isolated hepatocytes obtained from fasted and alloxan diabetic rats. Similar increases in the incorporation of 14C-bicarbonate into glucose was also observed. Net glucose production in isolated hepatocytes was also compared to other in vitro preparations. Net glucose production was much higher (2-5 fold) in isolated hepatocytes than that reported previously for liver slices or perfused liver. Studies on glycogen and protein synthesis show a 2 fold stimulation in the incorporation of 1-14C-glucoase into glycogen and U-14C-leucine into protein by the addition of 100 muU of insulin to isolated hepatocytes.
采用胶原酶体外灌注技术分离肝细胞。研究了从进食、禁食和四氧嘧啶糖尿病大鼠分离得到的肝细胞中的净葡萄糖生成情况。从禁食和四氧嘧啶糖尿病大鼠分离得到的肝细胞中，来自丙氨酸、丙酮酸和果糖的净葡萄糖生成增加了2至5倍。还观察到14C - 碳酸氢盐掺入葡萄糖的情况有类似增加。还将分离肝细胞中的净葡萄糖生成与其他体外制剂进行了比较。分离肝细胞中的净葡萄糖生成比先前报道的肝切片或灌注肝脏中的净葡萄糖生成高得多（2至5倍）。糖原和蛋白质合成研究表明，向分离的肝细胞中添加100微单位胰岛素可使1 - 14C - 葡萄糖掺入糖原以及U - 14C - 亮氨酸掺入蛋白质的过程受到2倍的刺激。
