对四氧嘧啶糖尿病大鼠分离肝细胞中糖异生和生酮作用的研究。

Studies on gluconeogenesis and ketogenesis in isolated hepatocytes from alloxan diabetic rats.

作者信息

Wagle S R, Ingebretsen W R

出版信息

Proc Soc Exp Biol Med. 1975 Dec;150(3):786-90. doi: 10.3181/00379727-150-39125.

PMID:1208599

Abstract

Gluconeogenesis and ketogenesis were studied in isolated hepatocytes obtained from normal and alloxan diabetic rats. Insulin treatment maintained near-normal blood glucose levels and caused an increase in glycogen deposition. The third day after insulin withdrawal the rats displayed a diabetic syndrome marked by progressive hyperglycemia and glycogen depletion. Net glucose production in liver cells isolated from alloxan diabetic rats progressively increased with time up to 72 hr after the last in vivo insulin injection. Maximal glucose production was observed at 72 hr with 10 mM alanine, lactate, pyruvate, or fructose. Glucose production decreased at 96 hr. The same pattern was observed with the incorporation of labeled bicarbonate into glucose. Ketogenesis in liver cells and hepatic lipid content also peaked at 72 hr.

摘要

对从正常大鼠和四氧嘧啶糖尿病大鼠分离得到的肝细胞中的糖异生和生酮作用进行了研究。胰岛素治疗维持了接近正常的血糖水平，并导致糖原沉积增加。停止胰岛素治疗后的第三天，大鼠出现了以进行性高血糖和糖原耗竭为特征的糖尿病综合征。从四氧嘧啶糖尿病大鼠分离的肝细胞中的净葡萄糖生成量在最后一次体内注射胰岛素后随时间逐渐增加，直至72小时。在72小时时，用10 mM丙氨酸、乳酸、丙酮酸或果糖观察到最大葡萄糖生成量。在96小时时葡萄糖生成量下降。将标记的碳酸氢盐掺入葡萄糖中也观察到相同的模式。肝细胞中的生酮作用和肝脏脂质含量在72小时时也达到峰值。