Boyer P D
FEBS Lett. 1975 Oct 15;58(1):1-6. doi: 10.1016/0014-5793(75)80212-2.
Acceptance of a membrane potential and/or a proton gradient as a possible means of transmitting energy from oxidations to ATP synthesis rests in part on a satisfactory hypothesis for how the potential or proton gradient could drive ATP synthesis. Recognition that energy input may drive ATP synthesis by change in binding of reactants at the catalytic site has led to the suggestions presented in this paper. These are that in oxidative phosphorylation and photophosphorylation, the requisite conformational changes may be coupled to exposure of charged groups to different sides of the membrane. The cycle of charged group exposure or movement may be driven by the membrane potential or, through protonation and deprotonation, may be coupled to proton translocation across the membrane. Effects of proton gradient and membrane potential may be additive. Similar conformational coupling suggestions may explain proton translocation coupled to ATP cleavage and active transport of metabolites coupled to membrane potential, proton gradients of ATP cleavage.
接受膜电位和/或质子梯度作为将氧化能量传递至ATP合成的一种可能方式，部分取决于关于电位或质子梯度如何驱动ATP合成的一个令人满意的假说。认识到能量输入可能通过催化位点反应物结合的变化来驱动ATP合成，引发了本文提出的观点。这些观点是，在氧化磷酸化和光合磷酸化过程中，必要的构象变化可能与带电基团暴露于膜的不同侧相关联。带电基团暴露或移动的循环可能由膜电位驱动，或者通过质子化和去质子化，可能与质子跨膜转运相关联。质子梯度和膜电位的作用可能是相加的。类似的构象偶联观点可能解释与ATP水解偶联的质子转运以及与膜电位、ATP水解的质子梯度偶联的代谢物主动转运。
