加拉帕戈斯海鬣蜥血红蛋白高分子量和低分子量形式的免疫化学特性

Immunochemical identity of the high and low molecular weight forms of Galapagos marine iguana hemoglobin.

Higgins P J

Sloan-Kettering Institute, Laboratory of Cell Biochemistry, New York, NY 10021.

Comp Biochem Physiol B. 1978;59(2):129-32. doi: 10.1016/0305-0491(78)90192-x.

Abstract

Two forms of Galapagos marine iguana methemoglobin, with molecular weights of 140,000 and 70,000 daltons, were identified in iguana RBC lysates by Sephadex G-200 molecular sieve fractionation. 2. The 140,000 dalton ferric hemoglobin was isolated by DEAE-Sephadex A-50 ion-exchange chromatography and found to be pure by electrophoretic and immunological criteria. 3. Immunochemical analyses revealed the high and low molecular weight hemoglobins to be antigenically identical.

摘要

通过葡聚糖凝胶G - 200分子筛分级分离，在鬣蜥红细胞裂解物中鉴定出两种形式的加拉帕戈斯海鬣蜥高铁血红蛋白，分子量分别为140,000和70,000道尔顿。2. 通过二乙氨基乙基葡聚糖A - 50离子交换色谱法分离出140,000道尔顿的高铁血红蛋白，经电泳和免疫学标准鉴定为纯品。3. 免疫化学分析表明，高分子量和低分子量血红蛋白在抗原性上相同。