用异源放射免疫分析法测定爬行动物血清中的降钙素。

Determination of calcitonin in reptilian serum by heterologous radioimmunoassay.

作者信息

Kline L W, Longmore G A

出版信息

Gen Comp Endocrinol. 1986 Jan;61(1):1-4. doi: 10.1016/0016-6480(86)90242-x.

Abstract

A heterologous radioimmunoassay for reptilian calcitonin using a commercially available kit for human calcitonin was used to determine circulating levels and brain levels of calcitonin in the green iguana, Iguana iguana. Cross-reactivity between anti-human calcitonin antibodies and iguana plasma was demonstrated. Removal of reptilian calcitonin from the plasma with affinity chromatography eliminated the cross-reactivity. Assays on blood samples indicated normal circulating levels of calcitonin to range between 600-975 pg/ml. Young female iguanas had higher blood levels than males. The use of this assay permits more detailed study of the secretion of calcitonin in reptiles in response to appropriate stimuli.

摘要

使用市售的人降钙素试剂盒对蜥蜴降钙素进行异源放射免疫分析，以测定绿鬣蜥（Iguana iguana）体内降钙素的循环水平和脑内水平。已证实抗人降钙素抗体与鬣蜥血浆之间存在交叉反应。用亲和色谱法从血浆中去除蜥蜴降钙素可消除交叉反应。对血样的分析表明，降钙素的正常循环水平在600 - 975 pg/ml之间。年轻雌性鬣蜥的血液水平高于雄性。这种分析方法的使用有助于更详细地研究蜥蜴体内降钙素在适当刺激下的分泌情况。