不同物种海藻糖酶的比较特异性

Comparative specificities of trehalases from various species.

Labat-Robert J, Baumann F C, Bar-Guilloux E, Robic D

Laboratoire de Chimie Biologique, E.R.A. No. 99 du C.N.R.S. Faculté des Sciences Pharmaceutiques et Biologiques, Paris, France.

Comp Biochem Physiol B. 1978;61(1):111-4. doi: 10.1016/0305-0491(78)90225-0.

Abstract

Using derivatives or non-symmetrical analogs of alpha,alpha-trehalose, we studied the catalytic specificities of trehalases from various species: Pseudomonas fluorescens, Melolontha vulgaris, porcine and human kidneys. 2. alpha,Beta-trehalose, beta,beta-trehalose, 6,6'dideoxy alpha,alpha-trehalose, alpha-D-xylopyranosyl alpha-D-xylopyranoside were shown to be neither substrates nor inhibitors. 3. 6'deoxy alpha,alpha-trehalose, alpha-D-glucopyranosyl alpha-D-xylopyranoside, alpha-D-allopyranosyl alpha-D-glucopyranoside and alpha-D-galactosyl alpha-D-glucopyranoside, which all possess an intact alpha-D-glucopyranosyl residue, were split by all these trehalases. 4. alpha-D-glucopyranosyl alpha-D-mannopyranoside, alpha,alpha-trehalosamine are competitive inhibitors. 5. These results show the importance of the primary alcohol group at C-6, of the equatorial configuration of the OH groups at C-2, C-3 and C-4 and of the modification of the structure at C-2 of the substrate for the catalytic activity.

摘要

我们使用α,α-海藻糖的衍生物或非对称类似物，研究了来自不同物种的海藻糖酶的催化特异性：荧光假单胞菌、普通鳃金龟、猪肾和人肾。2. α,β-海藻糖、β,β-海藻糖、6,6'-二脱氧α,α-海藻糖、α-D-吡喃木糖基α-D-吡喃木糖苷既不是底物也不是抑制剂。3. 6'-脱氧α,α-海藻糖、α-D-吡喃葡萄糖基α-D-吡喃木糖苷、α-D-阿洛吡喃糖基α-D-吡喃葡萄糖苷和α-D-半乳糖基α-D-吡喃葡萄糖苷，它们都具有完整的α-D-吡喃葡萄糖基残基，可被所有这些海藻糖酶分解。4. α-D-吡喃葡萄糖基α-D-甘露吡喃糖苷、α,α-海藻糖胺是竞争性抑制剂。5. 这些结果表明，C-6位的伯醇基团、C-2、C-3和C-4位OH基团的平伏构型以及底物C-2位结构的修饰对催化活性很重要。