文献检索，用中文搜 PubMed

Suppr 超能文献

核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Scott W N, Skipski I

Department of Physiology, Mount Sinai School of Medicine, City University of New York, New York 10029.

Comp Biochem Physiol B. 1979;63(3):429-35. doi: 10.1016/0305-0491(79)90273-6.

Two forms of carbonic anhydrase, having isoelectric points of 6.1 and 5.8, were purified from erythrocytes of the toad, Bufo marinus, and the presence of a third form, pI = 5.4, was demonstrated. 2. Each of the two purified isozymes catalyzed the hydration of CO2 and the hydrolysis of nitrophenyl acetate esters at rates characteristic of Type C (or high-activity) forms of carbonic anhydrase. 3. Both forms of the erythrocyte enzyme have similar molecular weights (approx 29,000), amino acid composition, sensitivity to acetazolamide, and kinetic properties. 4. The epithelium of the toad's urinary bladder also was found to contain significant amounts of carbonic anhydrase, which appears by isoelectric focusing to be indistinguishable from the enzyme isolated from the erythrocyte.

从海蟾蜍（Bufo marinus）的红细胞中纯化出两种等电点分别为6.1和5.8的碳酸酐酶，并证实存在第三种形式，其等电点为5.4。2. 两种纯化的同工酶各自催化二氧化碳的水合作用以及硝基苯乙酸酯的水解反应，其速率具有C型（或高活性）碳酸酐酶的特征。3. 红细胞酶的两种形式具有相似的分子量（约29,000）、氨基酸组成、对乙酰唑胺的敏感性以及动力学特性。4. 还发现蟾蜍膀胱上皮中含有大量碳酸酐酶，通过等电聚焦显示其与从红细胞中分离出的酶无法区分。

Scott W N, Skipski I

Department of Physiology, Mount Sinai School of Medicine, City University of New York, New York 10029.

Comp Biochem Physiol B. 1979;63(3):429-35. doi: 10.1016/0305-0491(79)90273-6.

Two forms of carbonic anhydrase, having isoelectric points of 6.1 and 5.8, were purified from erythrocytes of the toad, Bufo marinus, and the presence of a third form, pI = 5.4, was demonstrated. 2. Each of the two purified isozymes catalyzed the hydration of CO2 and the hydrolysis of nitrophenyl acetate esters at rates characteristic of Type C (or high-activity) forms of carbonic anhydrase. 3. Both forms of the erythrocyte enzyme have similar molecular weights (approx 29,000), amino acid composition, sensitivity to acetazolamide, and kinetic properties. 4. The epithelium of the toad's urinary bladder also was found to contain significant amounts of carbonic anhydrase, which appears by isoelectric focusing to be indistinguishable from the enzyme isolated from the erythrocyte.

从海蟾蜍（Bufo marinus）的红细胞中纯化出两种等电点分别为6.1和5.8的碳酸酐酶，并证实存在第三种形式，其等电点为5.4。2. 两种纯化的同工酶各自催化二氧化碳的水合作用以及硝基苯乙酸酯的水解反应，其速率具有C型（或高活性）碳酸酐酶的特征。3. 红细胞酶的两种形式具有相似的分子量（约29,000）、氨基酸组成、对乙酰唑胺的敏感性以及动力学特性。4. 还发现蟾蜍膀胱上皮中含有大量碳酸酐酶，通过等电聚焦显示其与从红细胞中分离出的酶无法区分。