Reich I M, Skipski I A, Scott W N
Biochim Biophys Acta. 1981 Sep 4;676(3):379-85. doi: 10.1016/0304-4165(81)90174-4.
A dual-label isotope technique was used to study the effects of aldosterone upon the incorporation of amino acids into proteins of the in vitro toad urinary bladder. Following labeling, the mucosal cells were disaggregated and the mitochondria-rich and granular cells were separated. Proteins with an elevated isotope ratio were found in a plasma membrane fraction (170 000, 110 000 and 85 000 daltons) and in the cytosol (36 000 and 6 000 daltons) of the preparations enriched in mitochondria-rich cells. These effects of aldosterone were blocked by cycloheximide. There was no evidence that aldosterone had induced the incorporation of labeled amino acids into carbonic anhydrase isolated from the soluble fraction by affinity chromatography. The results suggest that the physiologic response of the toad bladder to aldosterone is related to the synthesis of both soluble and plasma membrane proteins.
采用双标记同位素技术研究醛固酮对体外培养的蟾蜍膀胱中氨基酸掺入蛋白质的影响。标记后，将黏膜细胞分散，分离出富含线粒体的细胞和颗粒细胞。在富含线粒体的细胞制剂的质膜部分（170000、110000和85000道尔顿）和胞质溶胶（36000和6000道尔顿）中发现同位素比率升高的蛋白质。醛固酮的这些作用被环己酰亚胺阻断。没有证据表明醛固酮能诱导通过亲和层析从可溶性部分分离出的碳酸酐酶掺入标记氨基酸。结果表明，蟾蜍膀胱对醛固酮的生理反应与可溶性蛋白和质膜蛋白的合成有关。
