Campobasso O, Pecora M, Palestro G, Cozzani C
Tumori. 1975 Jan-Feb;61(1):17-28. doi: 10.1177/030089167506100103.
Plain paraffin was pellets and pellets containing 1% and 5% 20-MCA were implanted in the bladder of 219 C57 X IF and Swiss mice of both sexes. One hundred and fifty four survived and were killed after 40-42 weeks; 56 had been implanted with plain pellet and 98 with pellet containing the carcinogen. Only one bladder tumour (1,8%) developed in the former, whereas 35 tumours (35.7%) were found in the latter group. When a pellet containing 20-MCA was used, the tumour incidence was related to the strain and sex of the animals, rather than to the amount of carcinogen in the pellet. As a matter of fact, irrespective of the concentration of 20-MCA, females C57 X IF gave a significantly higher tumour yiels (87.5%) than males and Swiss mice of both sexes. It is concluded that when bladder implantation is used to induce tumours in mice, the results must be strictly referred to strain and sex. Besides tumours, areas of marked epithelial hyperplasia were seen in bladders implanted with either plain pellet or pellet containing 20-MCA. The possible meaning of this hyperplasia is briefly discussed and the need for further investigation stressed.
将普通石蜡制成丸粒，并将含有1%和5% 20 - 甲基胆蒽（20 - MCA）的丸粒植入219只C57 X IF和瑞士品系的雌雄小鼠膀胱中。154只小鼠存活下来，并在40 - 42周后处死；其中56只植入普通丸粒，98只植入含致癌物的丸粒。前者仅出现1例膀胱肿瘤（1.8%），而后者发现35例肿瘤（35.7%）。当使用含20 - MCA的丸粒时，肿瘤发生率与动物的品系和性别有关，而与丸粒中致癌物的含量无关。事实上，无论20 - MCA的浓度如何，C57 X IF雌性小鼠的肿瘤发生率（87.5%）显著高于雄性以及瑞士品系的雌雄小鼠。得出的结论是，当采用膀胱植入法在小鼠中诱导肿瘤时，结果必须严格参照品系和性别。除肿瘤外，在植入普通丸粒或含20 - MCA丸粒的膀胱中均可见明显的上皮增生区域。简要讨论了这种增生的可能意义，并强调了进一步研究的必要性。
