Stefanova M G, Balimezov I A
Vet Med Nauki. 1975;12(6):20-7.
Radioactive iron was used to follow up some hematologic indices in birds infected with irradiated spirochetes of the Borrelia anderina species. Used were a total of 90 cockerels, aged two months, divided into three groups. Ist group - cockerels inoculated with spirochetes that had been gamma-irradiated at the rate of 40000 R; IInd group-cockerels inoculated with untreated spirochetes; and IIIrd group - normal cockerels. The infective material consisted of strain Rouen spirochetes of the Pamoukchii serotype. Radiometric studies were also carried out to find out to what extent radioactive iron was incorporated in the erythrocytes and was deposited in the liver, spleen, and marrow of the investigated birds. Classical methods of investigation were employed to determine the erythrocyte, leukocyte, and thrombocyte counts, the hemoglobin content (after Sahli) as well as the erythrocyte pack after the method of Todorov. It was found that the inoculation of gamma-treated spirochetes led to sub-clinical changes in the hemopoiesis of the cockerels. Such changes, however, were negligible and were rapidly overcome by the birds. So they could successively be referred to in the specific immunoprophylaxis of spirochetosis. 59FeCl3 can be successfully applied in the study of anemia in birds caused by sbirochetosis.
用放射性铁来追踪感染了经辐照的安德列亚疏螺旋体（Borrelia anderina）的禽类的一些血液学指标。总共使用了90只两个月大的小公鸡，分为三组。第一组——接种以40000伦琴剂量进行γ辐照的螺旋体的小公鸡；第二组——接种未处理螺旋体的小公鸡；第三组——正常小公鸡。感染材料为由帕穆基血清型的鲁昂螺旋体菌株组成。还进行了放射性测量研究，以查明放射性铁在被研究禽类的红细胞中掺入以及在肝脏、脾脏和骨髓中沉积的程度。采用经典的研究方法来测定红细胞、白细胞和血小板计数、血红蛋白含量（萨利法）以及托多罗夫法测定的红细胞压积。发现接种经γ处理的螺旋体导致小公鸡造血出现亚临床变化。然而，这些变化微不足道，禽类能迅速克服。因此，它们可相继用于螺旋体病的特异性免疫预防。59FeCl3可成功应用于研究由螺旋体病引起的禽类贫血。
