[Demonstration of isoenzymes of human serum N-acetyl-beta-D-hexosaminidases, during pregnancy].

The N-acetyl-beta-D-hexosaminidase activity (EC 3.2.1.30) in human serum during pregnancy is increased. A polyacrylamide gel electrophoresis, without sodium dodecyl sulfate-uree, of the total serum allowed after incubation with the N-acetyl-beta-D-glucosaminide (6-bromo-2-hydroxy-3-naphthoyl-O-anisidine) coupled with the diazonium salt of O-amino-azotoluene to localise two zones of enzymatic activity. An electrophoresis of the same samples in exponential gradient polyacrylamide gel, shows 6 to 8 zones of different enzymatic activities.