Likhite V V, Wolff E
Oncology. 1975;32(5-6):327-32. doi: 10.1159/000225084.
Two malignant tumors of the human gastrointestinal tract (obtained surgically) have been cultivated in vitro in long-term organotypic tissue culture. Of these line AZ110 (maintained for 6 years) originated from a primary adenocarcinoma of the ascending colon, whereas line Z200 (grown for 14 years) originated from a hepatic metastatic nodule of a gastric adenocarcinoma. Extracts of these tumors and comparable surgical specimens were evaluated quantitatively for carcinoembryonic antigen (CEA) using radioimmunoassay. Both cultured lines exhibited CEA levels that were somewhat higher than comparable surgical specimens.
已对两个人类胃肠道恶性肿瘤（通过手术获取）进行了长期器官型组织培养。其中，AZ110细胞系（已维持6年）源自升结肠原发性腺癌，而Z200细胞系（已培养14年）源自胃腺癌的肝转移结节。使用放射免疫分析法对这些肿瘤提取物和类似手术标本中的癌胚抗原（CEA）进行了定量评估。两个培养细胞系的CEA水平均略高于类似手术标本。
