Drouet J
Rev Fr Transfus Immunohematol. 1975 Jun;18(2):217-25. doi: 10.1016/s0338-4535(75)80021-3.
The different radioimmunoassay technics routinely used for detection of HBs Antigen are Ausria I, Ausria II, RIA and CLB/RIA. Classification of these techniques either liquid or solide phase, is realized by nature of the tracer (Antigen or Antibody radioiodinated). The sensitivity and the specificity are presented as the comparison with other reactions. There is no significative difference on 1089 tested samples between Ausria I and RIA. The percentage of false positives in RIA or CLB/RIA reactions is 1.5% when the incubation is at 20 degrees C and 1% when it is at 37 degrees C. Advantages, disadvantages, cost prices, automation and possibility of computer treatment are discussed. The utilization of reference sera is proposed and the problem of the utilization en France of the radioactive products is approached.
常规用于检测乙肝表面抗原的不同放射免疫分析技术有Ausria I、Ausria II、RIA和CLB/RIA。这些技术根据示踪剂（放射性碘化抗原或抗体）的性质分为液相或固相。其灵敏度和特异性是通过与其他反应进行比较得出的。在1089份检测样本中，Ausria I和RIA之间没有显著差异。当孵育温度为20摄氏度时，RIA或CLB/RIA反应中的假阳性率为1.5%；当孵育温度为37摄氏度时，假阳性率为1%。文中讨论了这些技术的优缺点、成本价格、自动化程度以及计算机处理的可能性。还提出了使用参考血清的建议，并探讨了法国放射性产品的使用问题。
