乙型肝炎抗原反向被动血凝试验与放射免疫测定法的比较。

Comparison of the reversed passive hemagglutination with radioimmunoassay methods for hepatitis B antigen.

作者信息

Leers W D, Kouroupis G M

出版信息

J Clin Microbiol. 1975 Jul;2(1):8-10. doi: 10.1128/jcm.2.1.8-10.1975.

DOI:10.1128/jcm.2.1.8-10.1975

PMID:1225932

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC274117/

Abstract

Radioimmunoassay for the detection of hepatitis B antigen has been accepted in many diagnostic laboratories now. The question of nonspecific positives has always been a matter of controversy. Two improved radioimmunoassay tests, namely Ausria II-125 by Abbott Laboratories and a radioimmunoassay method by Connaught Laboratories Limited (Hebria), were compared with the original Ausria 125I. Included in the comparison was the reversed passive hemagglutination test (Auscell, Abbott). Five hundred sera of clinical patients were tested. Fifty-five or 11% were found to have hepatitis B antigen. Three tests, Ausria 125I, Ausria II-125, and Hebria showed the same number of positive sera, whereas Auscell missed one. However, Ausria 125I indicated two additional false positives. Dilution experiments, however, indicated that Ausria II-125 and Hebria were the most sensitive tests, with the reversed passive hemaglutination showing the least sensitivity. Therefore, the new Ausria II-125 and the Hebria radioimmunoassay tests are preferable in view of their sensitivity and specificity.

摘要

用于检测乙型肝炎抗原的放射免疫测定法目前已被许多诊断实验室所采用。非特异性阳性的问题一直存在争议。将两种改进的放射免疫测定法，即雅培实验室的Ausria II - 125和康诺特实验室有限公司（Hebria）的放射免疫测定法，与原来的Ausria 125I进行了比较。比较中还包括反向被动血凝试验（Auscell，雅培）。对500例临床患者的血清进行了检测。发现55例（即11%）患者有乙型肝炎抗原。Ausria 125I、Ausria II - 125和Hebria这三种检测方法显示出相同数量的阳性血清，而Auscell漏检了1例。然而，Ausria 125I还显示出另外2例假阳性。不过，稀释试验表明，Ausria II - 125和Hebria是最敏感的检测方法，反向被动血凝试验的敏感性最低。因此，鉴于其敏感性和特异性，新的Ausria II - 125和Hebria放射免疫测定法更可取。