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[辐照马铃薯的储存及烹饪加工对其提取物细胞遗传活性的影响]

[Influence of the storage and culinary processing of irradiated potatoes on the cytogenetic activity of extracts obtained from them].

作者信息

Osipova I N, Shillinger Iu I, Zaĭtsev A N

出版信息

Vopr Pitan. 1975 Jul-Aug(4):54-7.

PMID:1229110
Abstract

Male-rats (25--27 g) were given perorally extracts separated from potato subjected to gamma-radiation in a dose of 10 krads (test groups) and from nonirradiated tubers (controls). The extracts were introduced for a period of one week, daily in an amount of 1 ml. The male from the test groups (each numbering 8--10 animals) received extracts of the raw potato stored for 4 months after irradiation and of the potato subjected to thermal treatment (cooking) after 1 day, 1 and 4 months of its storage. The frequency of chromosomal aberrations in the bone marrow cells was determined by the anaphase method. Altogether about 34 thous. cells (500-600 from each animal) were counted. The results testified to a significantly reduced frequency of chromosomal aberrations (bridges and fragments) occurring in the bone marrow cells of the mice which received extracts from the raw stored irradiated potato and from thermally treated freshly irradiated tubers, as compared to extracts obtained from the raw freshly irradiated potatoes. The extracts of irradiated potato cooked after 1 and 4 month of storage did not display any mutagenic properties.

摘要

给雄性大鼠(体重25 - 27克)经口灌胃给予从经10千拉德剂量γ射线辐照的马铃薯中分离出的提取物(试验组)以及从未经辐照的块茎中分离出的提取物(对照组)。提取物连续给予一周,每日剂量为1毫升。试验组(每组8 - 10只动物)的雄性大鼠接受辐照后储存4个月的生马铃薯提取物以及辐照后1天、1个月和4个月储存的经过热处理(烹饪)的马铃薯提取物。采用后期分析法测定骨髓细胞中的染色体畸变频率。总共计数了约34000个细胞(每只动物500 - 600个)。结果表明,与从未经辐照的新鲜马铃薯获得的提取物相比,接受辐照后储存的生马铃薯提取物以及辐照后新鲜马铃薯经热处理后的提取物处理的小鼠骨髓细胞中,染色体畸变(桥和片段)的频率显著降低。辐照后1个月和4个月储存的马铃薯烹饪后的提取物未显示出任何诱变特性。

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